The Expression and Clinical Significance of RUNX3 Protein in ...

Abstract

Objective To explore the expression of tumor suppressor gene RUNX3 protein in gastric cancer, colorectal cancer tissues, and comparative analysis of both the relevance and clinical significance. To further understand the tumor suppressor gene RUNX3?s role in the digestive tract, for their theoretical research, clinical diagnosis and treatment help.Methods Collected 52 cases of resected gastric cancer patients and the same period 41 patients with rectal cancer surgical specimens, to study which tumor suppressor gene RUNX3 protein in gastric cancer, rectal carcinoma study:? Gastric cancer, colorectal cancer organizations and the corresponding adjacent normal tissue morphology change were observed by HE staining;?Tumor suppressor gene RUNX3 protein in gastric cancer, colorectal cancer tissues and clinical correlation were detected by immunohistochemical SP method;?Connection of RUNX3 protein in gastric cancer, colorectal cancer tissue were contacted by computer image analysis;?UNX3 protein in gastric cancer, rectal carcinoma were detected by Western blot;?Immunohistochemical analysis of data mean optical density value, and Western blot gray value were processed by Statistical software SPSS for Windows13.0 image.Results?HE staining showed normal tissue adjacent to gastric cancer, rectal wall from outside to the inside of four tiers, mucous layer, submucosa, muscle, membrane, mucous membrane layer was divided into epithelium, lamina propria, muscularis mucosa. HE staining showed histological type adenocarcinoma mainly common with tubular adenocarcinoma in gastric cancer; carcinoma of rectum histological type, the well-differentiated tubular adenocarcinoma and papillary adenocarcinoma more common.?Immunohistochemical staining showed that 52 cases of gastric cancer of corresponding adjacent normal tissues could be expression of RUNX3 protein, mainly located in the surface mucous cells and gastric glands in the cytoplasm; 32 cases (61.5%) RUNX3 protein expression in 52 cases of gastric carcinoma and the lower level of differentiation, the less positive expression, and there was coloring the cytoplasm and nuclei of heterogeneity; 38 cases (92.7%) in 41 cases of rectal mucosa adjacent to the corresponding normal tissue expression of RUNX3 protein, located in the cytoplasm of columnar epithelial cells,19 cases (46.3%) in 41 cases of rectal cancer tissues were positive, which lower than the positive rate of RUNX3 protein in gastric cancer (P

Source: http://www.res-medical.com/oncology/58942

dos santos waterboarding boxing news boxing andy dalton corporal kelsey de santis corporal kelsey de santis